In-field evaluations of bacterial presence in minutes
Our robust technology delivers accurate in-field evaluations of bacterial presence and activity minutes after sampling. This secures a rapid geographical bacteria survey on an entire field or production equipment sequence to determine the extent of bacterial contamination within one day. Fast, on-site results can characterize biocide effectiveness, evaluate the biostability of the water, and monitor the microbiology that may cause corrosion, souring, or fouling.
Results can be obtained in as little as 10 minutes with a sensitivity < 100 CFU/ml. The technology is applicable across a range of water types and sources, and the analysis is not compromised by the presence of chemicals, tannins, algae, clays, or other materials.
How Bactiquant’s technology works
Water samples are filtered through a filter 0.22, where the bacteria are collected on the surface of the membrane, eliminating potential background interferents.
The enzyme substrate is added directly to the filter house. The substrate reacts with a naturally occurring bacterial enzyme, releasing a fluorescent compound. The reaction time is approximately 15–30 min depending on the application.
Enzyme activity with fluorescence is measured using a handheld fluorometer. The more fluorescence produced, the more bacteria are present in the sample.
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Metal corrosion associated with the growth and reproduction of microorganisms is known as microbiologically influenced corrosion (MIC). MIC causes damage to metal surfaces in several water-based industries including drinking water distribution, cooling water equipment, sewage treatment, underground pipes, bilges, piping, and tanks of maritime vessels. MIC is extensively seen in the oil and gas industry.
Product Number: 51323-19047-SG
Author: M, Miller, A, Steffensen, J. Fajt
Publication Date: 2023
Controlling bacteria in Oil & Gas ops is crucial for flow assurance and system integrity. Bacteria become more resistant over time, like leveling up in a game. Detecting high-skill bacteria quickly is vital for HSE, corrosion prevention, and efficiency. Traditional methods take too long (24 hours to 28 days). A portable fluorometric technology identifies and monitors bacterial contamination in real-time, helping address issues promptly, such as a failed biocide injection pump.
Paper Number: SPE-210349-MS
Author: M Miller, A Steffensen, S Elleskov, E Vilain
Publication Date: September 2022
Tetrakis(hydroxymethyl)-phosphonium Sulfate (THPS) is a very common active ingredient in oil and gas biocides. While product labels provide broad guidelines application dosing the lowest effective dose of THPS is difficult to determine. Site water chemistry and bacteria biology variability will affect the dose need to achieve the desired level of bacteria population control. For these reasons biocide dose response studies are commonly conducted on solutions containing bacteria to determine the effect of treatments before application.